Journal: Frontiers in Cell and Developmental Biology

Article Title: Rapamycin Promotes the Expansion of Myeloid Cells by Increasing G-CSF Expression in Mesenchymal Stem Cells

doi: 10.3389/fcell.2022.779159

Figure Lengend Snippet: Rapamycin increased G-CSF expression in MSCs in vitro (A) Experiment design for MSCs treated by rapamycin in vitro (B–C) Fluorescence intensity of p-S6 and p-4E-BP1 in MSCs treated by rapamycin or ethanol in vitro (D) mRNA expression of G-CSF in MSCs treated by rapamycin or ethanol in vitro (E) Experiment design for the co-culture of MSC and LK + cells (F) The protein levels of G-CSF in the cell lysates of MSC treated by rapamycin or ethanol (G) The G-CSF level in the media of MSC treated by rapamycin or ethanol (H) The percentage (Left panel) and absolute number (right panel) of myeloid cells after coculturing with MSCs treated by rapamycin + G-CSF antibody, singel rapamycin, or ethanol.

Article Snippet: For the G-CSF neutralization experiment, G-CSF antibody (R&D, MAB406-SP, United States) was added to the coculture system at 2 nM.

Techniques: Expressing, In Vitro, Fluorescence, Co-Culture Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Rapamycin Promotes the Expansion of Myeloid Cells by Increasing G-CSF Expression in Mesenchymal Stem Cells

doi: 10.3389/fcell.2022.779159

Figure Lengend Snippet: Rapamycin prompted MSCs to produce more G-CSF in vivo (A) Experiment design of MSCs isolated from mice treated with rapamycin or vehicle by i. p., n = 3, days = 30, dose = 4 mg/kg/day (left panel). Fluorescence intensity of p-S6 and p-4E-BP1 in MSCs treated by rapamycin or vehicle by i. p (right and middle panels) (B) mRNA relative expression of G-CSF in MSCs from mice treated with rapamycin or vehicle by i. p. (C) Experiment design of MSCs cultured in vitro (left panel). mRNA relative expression of G-CSF in MSCs after culture (right panel) (D–E) Relative expression of G-CSF、TNF- α and M-CSF in serum from mice treated with rapamycin or vehicle by Milliplex multifactor detection technology (F) Experiment design of MSCs isolated from mice treated with rapamycin or ethanol by p. o., n = 3, days = 30, dose = 2 mg/kg/day (left panel). Relative mRNA expression of G-CSF in MSCs of two groups (right panel).

Article Snippet: For the G-CSF neutralization experiment, G-CSF antibody (R&D, MAB406-SP, United States) was added to the coculture system at 2 nM.

Techniques: In Vivo, Isolation, Fluorescence, Expressing, Cell Culture, In Vitro